Reginald C. Barker was born in Winnipeg, Manitoba, Canada his family moved to Scotland when he was an infant and then to the United States. Living in California, Barker wrote, produced, and acted in his first play at the age of sixteen following which he acted and handled stage manager duties with a traveling stock company. At age nineteen, he went to New York City where he worked as a stage manager for Henry Miller. Barker made his Broadway acting debut in 1910 in the Shubert brothers production of "Mary Magdalene" written by Maurice Maeterlinck.
Fascinated by the fledgling film business, Barker soon joined the Bison Motion Pictures division of the New York Motion Picture Company. At the company's studio/ranch in California, he worked under film producer and screenwriter Thomas H. Ince.
Acting was not Barker's forte and he trained as an assistant director until 1912 when he directed his first film, a twenty minute western titled "On the Warpath" starring Art Acord. Barker went on to direct more than eighty films, including the acclaimed 1915 American Civil War drama The Coward. That same year he directed The Italian but because Thomas H. Ince was notorious for credit-
During his career, Reginald Barker directed early stars such as Geraldine Farrar, William S. Hart, Sessue Hayakawa, Gladys Brockwell, Hoot Gibson, Willard Mack, and Myrna Loy. In his first talkie, "The Toilers" (1928) he directed Douglas Fairbanks Jr.. Barker made his last film in 1935. Titled "The Healer," it starred Ralph Bellamy, Karen Morley and Mickey Rooney.
Reginald Barker retired to Pasadena, California where he and his wife operated a gift shop until his passing from a heart attack in 1945.